19 sites (16 wetlands and 3 rivers) with different physicochemical characteristics and distributed along three rivers (Ain, Doubs, and Loue) were sampled. The abundances of some gram-negative bacteria (Escherichia coli, extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa) were determined.
Data acquisition : from Apr 2015 to Mar 2016
Data provision : 26 Oct 2021
Metadata record :
Creation : 22 Oct 2021
Update : 26 Oct 2021
Additional information :
Data acquired during Charles Henriot's PhD work, supervised by Pr. Didier Hocquet (University Professor - Hospital practitioner at the Chrono-environment laboratory) and Dr. Gudrun Bornette (Researcher in ecology at the Chrono-environment laboratory)
University: licence, University: master, Research
Formats : application/vnd.ms-excel, application/vnd.oasis.opendocument.spreadsheet, text/csv
Data acquisition methods :
- Observational data :
Samples of water (250 ml) were collected in sterile conditions and stored at 4◦C before analysis, which occurred within 8 h of collection. The total concentrations of E. coli, K. pneumoniae and
P. aeruginosa were measured as previously described (Slekovec et al. 2012; Brechet ´ et al. 2014). Aliquots of water (100 ml) were filtered through 0.45-μm membranes, which were then placed onto a Drigalski agar plate to measure the total E. coli and K. pneumoniae concentrations (Oxoid), a Cetrimide agar plate to measure the P. aeruginosa concentration (Bio-Rad) and a chromID ESBL agar plate to measure the concentration of E. coli producing extended-spectrum beta-lactamases (ESBL), which are resistant to β-lactam antibiotics. When we suspected high bacterial concentrations, we plated 500 μl an additional of sample on the selective media. The plates were incubated for 24 h at 37◦C. Colonies were counted and classified according to their phenotype. Each type of colony was identified using a MALDITOF MS spectrometer (Microflex, Bruker Daltonics, Bremen, Germany) according to the manufacturer’s recommendations. For each morphotype of E. coli growing on chromID ESBL, the production of ESBL was detected using a synergy test following the recommendations of the Antibiogram Committee of the French Society for Microbiology (CA-SFM, http://www.sfm-microbiologie.org/
, last consultation: 10 November 2018).